Invitrogen™ S100A1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1932

Catalog No. PA1932

Description

PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein. PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

Specifications

• Antigen: S100A1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: S100A1
• Gene Alias: AI266795; Protein S100-A1; S100; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100a; S100A1; S100-alpha
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 106504218, 20193, 295214, 528735, 6271
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20mg/mL BSA and 0.1% sodium azide
• Gene Accession No.: P02639, P23297, P35467, P56565
• Gene Symbols: S100A1
• Immunogen: Purified S100-alpha protein from human pectoral muscle cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG