Invitrogen™ Sambucus Nigra (Elderberry Bark) Lectin (SNA, EBL), fluorescein (FITC)

Description

Bright-green SNA-fluorophor conjugate

• Sambucus Nigra (Elderberry Bark) Lectin (SNA, EBL), fluorescein (FITC), is a bright-green SNA-fluorophor conjugate. SNA belongs to the group of type 2 ribosome-inactivating proteins and is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. SNA can induce apoptosis through the caspase-dependent pathway at low concentrations.
• This effect depends on the carbohydrate binding of the B-chain and is independent from the catalytic activity of the A-chain. In colon cancers, it has been reported that 90% of carcinomas express increased levels of sialic acid, linked to galactose residues on the surface of tumor cells, that is detectable with SNA.
• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: sialic acid
• Inhibiting/eluting sugar: 500 mM lactose
• Blood group specificity: non-specific, A > B, O
• Unconjugated lectin molecular weight: 140 KDa
• Immunofluorescence working concentration: 5–20 μg/mL
• SNA-FITC is provided at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM Ca²⁺
• A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.

Specifications

• Quantity: 2 mg
• Source: Elderberry
• Conjugate: Fluorescein
• Content And Storage: When stored at or below 2–8°C in the dark, the product is stable for 6 months after reciept.
  
  
• Label Type: Classic Dyes
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Form: Solution
• Protein Subtype: Lectins

For Research Use Only. Not for use in diagnostic procedures.