Invitrogen™ SARS-CoV-2 Spike Protein (RBD) Chimeric Recombinant Rabbit Monoclonal Antibody (P05DHuRb), Alexa Fluor™ 647, eBioscience™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 51649182

Catalog No. 51-649-182

Description

Description: This antibody has a human Fab with a kappa light chain and Rabbit Fc. It recognizes the RBD domain of SARS CoV2 Spike protein. Applications Tested: This P05DHuRb Alexa Fluor™ 647 conjugate has been tested by flow cytometric analysis of HEK 293 cells transfected with a vector encoding full length spike protein from SARS CoV2. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation:633-647 nm; Emission: 668 nm; Laser: Red Laser Filtration: 0.2 μm post-manufacturing filtered.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

Specifications

• Antigen: SARS-CoV-2 Spike Protein (RBD) Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS, Proprietary buffer with 0.09% sodium azide; pH 7.2
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG κ

• Applications: Flow Cytometry
• Clone: P05DHuRb
• Conjugate: Alexa Fluor 647
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Immunogen: This antibody was developed using computationally derived variants of SARS-CoV antibody structures and is shown to bind Spike Protein S1 subunit (RBD) of SARS CoV2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid