Invitrogen™ SARS-CoV-2 Spike Protein (RBD) (B.1.617.2) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88488	100 μg

Catalog No. PIRP88488 Supplier Invitrogen™ Supplier No. RP88488

Description

Recombinant Protein

Reconstituted with sterile deionized water to 0.25mg/mL. It is recommended that the protein be aliquoted for optimal storage. The mutations were identified in the SARS-CoV-2 varient known as B.1.617.2.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

Specifications

• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Molecular Weight (g/mol): 36.9 kDa
• Name: SARS-CoV-2 Spike Protein (RBD) (B.1.617.2) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein RBD
• Biological Activity: Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag), the EC50 of SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag) is 4-24 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: SARS-CoV-2 spike RBD (Accession # YP_009724390.1), amino acids Arg319-Phe541 with L452R,T478K substitutions and a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag), the EC50 of SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag) is 4-24 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.