Invitrogen™ SARS-CoV-2 Spike Protein S1 Fc Chimera Recombinant Protein

Catalog No.	Quantity
PIRP88437	100 μg

Catalog No. PIRP88437 Supplier Invitrogen™ Supplier No. RP88437

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (667 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

Specifications

• Concentration: 0.25 mg/mL
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Molecular Weight (g/mol): 101.4 kDa
• Name: SARS-CoV-2 Spike Protein S1 Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein S1
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1, mFc Tag) (RP-88437), the EC50 of 2019-nCoV Spike Protein (S1, mFc Tag) is 90-240 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: SARS-CoV-2 spike S1, amino acids Val16-Arg685 (Accession # YP_009724390.1) with a C-terminal mouse IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1, mFc Tag) (RP-88437), the EC50 of 2019-nCoV Spike Protein (S1, mFc Tag) is 90-240 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE.