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Sartorius™ Vivapure™ LentiSELECT 500 Purification and Concentration Kit

Fast purification of up to 3 x109 viral particles

Manufacturer:  Sartorius™ VSLVPQ500

Catalog No. 14-558-565


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Description

Description

Vivapure™ LentiSELECT 500 is optimally suited for VSV-G pseudotyped lentivirus purification from up to 500mL cell culture and contains all reagents and devices necessary for purifying up to 2-5 x 109 infective particles.

The whole purification procedure is simply operated by a laboratory pump, which min imizes hands-on time. Unlike conventional purification methods as ultracentrifugation, Vivapure LentiSELECT 500 offers a fast and simple solution for purifying VSV-G pseudotyped lentiviruses making expensive purification equipment like ultracentrifuges redundant.

Typically, for a normal yielding vector, a 500mL cell culture purified using this method will yield up to 2–5 x 109 infective particles in 1mL (total volume 1mL).

  • Fast
  • Non-toxic
  • Better purity than pelleting by ultracentrifugation
  • Convienient
  • Cost effective

Specifications

Specifications

Vivapure™ LentiSELECT 500 kit
Lenti select unit 1, sarto pore 2 150 clarifying filter 1, 50mL syringe 1, tubing set 1, 30mL Load Buffer (10X), 170mL Wash Buffer (1X), 30mL Elution Buffer (1X), vivaspin 20, 100kDa MWCO 2, technical data sheet 1 each for kit and vivaspin
3 hr.
Virus purification
500mL of Lentivirus
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