Agilent Technologies™ Seahorse XFp Glycolytic Rate Assay Kit

Description

Seahorse XFp Glycolytic Rate Assay Kit containing six single-use pouches, each with rotenone/antimycin A and 2-deoxy-D-glucose. For use with Seahorse XF HS Mini and XFp analyzers. XF pheno-red-free media with low buffer capacity are required to perform this assay.

• Provides a precise measure of glycolytic rate by calculating and subtracting mitochondrial-produced acidification
• Detects metabolic switches (such as the Warburg effect) by reporting the relative activity of glycolysis and mitochondrial respiration
• Measures the effects of metabolic modulators on glycolytic rates within a single assay well
• Assay is validated and best performed with Agilent™ Seahorse XF DMEM medium, pH 7.4 or Agilent™ Seahorse XF RPMI medium, pH 7.4
• Kit contains a Rotenone/Antimycin A mix to block mitochondrial activity (for calculation of mitochondrial acidification) and 2-DG (to inhibit glycolysis) as an internal control
• Single-use vials assure quality and quantity every time and are now easier to open
• Upload your assay result file to your Agilent™ Seahorse Analytics account to calculate and report parameters of the XF Glycolytic Rate assay, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
• Not compatible with Agilent™ Seahorse XF24 and XF24-3 Analyzers

Specifications

• Detection Method: Fluorescence
• Includes: Lyophilized Reagents
• Quantity: 1 Kit
• Type: Glycolytic Rate Assay Kit