Invitrogen™ SEC16A Polyclonal Antibody

Catalog No.	Quantity
PIPA552182	100 μL

Catalog No. PIPA552182 Supplier Invitrogen™ Supplier No. PA552182

Description

Rabbit Polyclonal Antibody

Immunogen sequence: PLPIPSNLFV PTPDAEEPQL PDGTGREGPA AARGLANPEP APEPKAPGDL PAAGGPPSGA MPFYNPAQ Highest antigen sequence identity to the following orthologs: Mouse - 46%, Rat - 47%.

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Specifications

• Antigen: SEC16A
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene: SEC16A
• Gene Accession No.: O15027
• Gene Alias: KIAA0310; p250; protein SEC16 homolog A; protein transport protein Sec16A; SEC16; SEC16 homolog A; SEC16 homolog A, endoplasmic reticulum export factor; SEC16A; SEC16L
• Gene Symbols: SEC16A
• Host Species: Rabbit
• Immunogen: Recombinant protein corresponding to Human SEC16A. Recombinant protein control fragment (Product #RP-89209).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 9919
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG