Invitrogen™ SEMA4A Monoclonal Antibody (KL-1 (aka SK31)), eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14100282

Catalog No. 50-127-79

Description

Description: The KL-1 (aka SK31) antibody reacts with mouse Sema4A, a class IV semaphorin originally identified as SemB. Sema4A is structurally similar to Sema4D/CD100. Sema4A is reported to be expressed by dendritic cells, B cells and activated T cells. Although published data suggested that Sema4A binds to Tim-2, a T cell surface receptor, it remains to be definitively shown that these two molecules have a receptor/ligand relation. It is suggested that Sema4a can provide costimulatory signals for T cells. This KL-1 antibody works in immunoblotting but requires non-reducing conditions. Applications Reported: This KL-1 (aka SK31) antibody has been reported for use in immunoblotting (WB). Applications Tested: The KL-1 (aka SK31) antibody has been tested by immunoblot analysis of mouse Sema4A transfected cells under nonreducing conditions. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.

Specifications

• Antigen: SEMA4A
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q62178
• Gene Symbols: SEMA4A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20351
• Content And Storage: 4°C
• Form: Liquid

• Applications: Western Blot
• Clone: KL-1 (aka SK31)
• Conjugate: Unconjugated
• Gene: SEMA4A
• Gene Alias: AI132332; CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; Sema4a; Semab; semaphorin 4A; semaphorin B; semaphorin-4A; Semaphorin-B; SemB; UNQ783/PRO1317
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a