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Invitrogen™ SEMA4A Monoclonal Antibody (KL-1 (aka SK31)), eBioscience™
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Catalog No. 5012779
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100 μg
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Catalog No. Quantity
50-127-79 100 μg
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Catalog No. 50-127-79 Supplier Invitrogen™ Supplier No. 14100282
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Description

Rat Monoclonal Antibody

Description: The KL-1 (aka SK31) antibody reacts with mouse Sema4A, a class IV semaphorin originally identified as SemB. Sema4A is structurally similar to Sema4D/CD100. Sema4A is reported to be expressed by dendritic cells, B cells and activated T cells. Although published data suggested that Sema4A binds to Tim-2, a T cell surface receptor, it remains to be definitively shown that these two molecules have a receptor/ligand relation. It is suggested that Sema4a can provide costimulatory signals for T cells. This KL-1 antibody works in immunoblotting but requires non-reducing conditions. Applications Reported: This KL-1 (aka SK31) antibody has been reported for use in immunoblotting (WB). Applications Tested: The KL-1 (aka SK31) antibody has been tested by immunoblot analysis of mouse Sema4A transfected cells under nonreducing conditions. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.
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Specifications

Antigen SEMA4A
Applications Western Blot
Classification Monoclonal
Clone KL-1 (aka SK31)
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene SEMA4A
Gene Accession No. Q62178
Gene Alias AI132332; CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; Sema4a; Semab; semaphorin 4A; semaphorin B; semaphorin-4A; Semaphorin-B; SemB; UNQ783/PRO1317
Gene Symbols SEMA4A
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 20351
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2a
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