Novus Biologicals™ Semaphorin 4D/CD100 Antibody Pair [HRP]

Catalog No.	Quantity
NB010233	15 Plates

Catalog No. NB010233 Supplier Novus Biologicals™ Supplier No. NBP27959715PLATES

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse Semaphorin 4D/CD100.

Specifications

• Assay Range: 47 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse Semaphorin 4D/CD100.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: A8, CD100 antigen, CD100M-sema-G, coll-4, FLJ33485, FLJ34282, FLJ39737, FLJ46484, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D, MGC169138, MGC169141, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D, semaphorin-4D
• Gene ID (Entrez): 10507
• Gene Symbol: SEMA4D
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL rabbit anti-Semaphorin 4D/CD100 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 1.0 mg/mL of rabbit anti-mouse Semaphorin 4D/CD100 monoclonal antibody.
  Standard: Each vial contains 150 ng of recombinant mouse Semaphorin 4D/CD100.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Immunology
• Storage Requirements: Storage is content dependent.
• Target: Semaphorin 4D/CD100
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.