Novus Biologicals™ Serpin E1/PAI-1 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Serpin E1/PAI-1.

Specifications

• Assay Range: 46.88 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Serpin E1/PAI-1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Endothelial plasminogen activator inhibitor, PAI1PAI-1, PAISerpin E1, PLANH1plasminogen activator inhibitor 1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1
• Gene ID (Entrez): 5054
• Gene Symbol: SERPINE1
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 125 ng of recombinant Human Serpin E1/PAI-1.
  Capture Antibody: 1 mg/mL of mouse anti-Human Serpin E1/PAI-1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human Serpin E1/PAI-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer
• Storage Requirements: Storage is content dependent.
• Target: Serpin E1/PAI-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.03125 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.