Invitrogen™ SF1 Monoclonal Antibody (2F5D10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549179

Catalog No. PIMA549179

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human SKOV-3 whole cell, human K562 whole cell, human THP-1 whole cell, human A549 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell. IHC: human bladder cancer tissue, human breast cancer tissue, human liver cancer tissue, human placenta tissue, human squamous metaplasia of the renal pelvis tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue. ICC/IF: A431 cell. Flow: A431 cell, Neuro-2a cell, C6 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: SF1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q15637, Q64213
• Gene Symbols: SF1
• Immunogen: E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2F5D10
• Conjugate: Unconjugated
• Gene: SF1
• Gene Alias: BBP; D11S636; M ZFM1; Mammalian branch point-binding protein; MBBP; MZFM; SF; SF1; Splicing factor 1; transcription factor ZFM1; WBP4; wu:fc09f06; ZCCHC25; ZFM1; Zfp162; Zinc finger gene in MEN1 locus; zinc finger protein 162; ZNF162
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117855, 22668, 7536
• Content And Storage: -20°C
• Form: Lyophilized