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Invitrogen™ SF1 Polyclonal Antibody
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Catalog No. PIPA579983
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100 μg
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PIPA579983 100 μg
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Catalog No. PIPA579983 Supplier Invitrogen™ Supplier No. PA579983
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Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human SK-OV-3 whole cell, human HepG2 whole cell, mouse NIH/3T3 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: mouse lung tissue, rat lung tissue, human placenta tissue, human rectal cancer tissue, mouse small intestine tissue, rat small intestine tissue. ICC/IF: A431 cell. Flow: A431 cell.

Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
TRUSTED_SUSTAINABILITY

Specifications

Antigen SF1
Applications ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 500 μg/mL
Conjugate Unconjugated
Formulation PBS with 4mg trehalose and 0.05mg sodium azide
Gene SF1
Gene Accession No. Q15637, Q64213
Gene Alias BBP; D11S636; M ZFM1; Mammalian branch point-binding protein; MBBP; MZFM; SF; SF1; Splicing factor 1; transcription factor ZFM1; WBP4; wu:fc09f06; ZCCHC25; ZFM1; Zfp162; Zinc finger gene in MEN1 locus; zinc finger protein 162; ZNF162
Gene Symbols SF1
Host Species Rabbit
Immunogen E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 117855, 22668, 7536
Target Species Human, Mouse, Rat
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
Isotype IgG
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