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Sigma Aldrich Fine Chemicals Biosciences SIG10 Chemically Competent
Supplier: Sigma Aldrich Fine Chemicals Biosciences CMC000140X40UL
The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.These cells are ideal for cloning and propagation of plasmid cosmid or fosmid clones and are highly efficient for routine cloning applications. They share the most useful genetic elements of standard cloning strains like DH5alpha(TM) DH10B(TM) JM109 TOP10 etc. and directly replace them in cloning protocols.They are are provided in 40 muL 80 muL and 160 muL aliquots each being sufficient for one two and four transformations respectively. The cells have a transformation efficiency of 1 x 109 cfu/mug The 96-well format are provided in aliquots of 20 muL per well and have a transformation efficiency of 1 x 108 cfu/mug.Genotypef- mcrA Delta(mrr-hsdRMS-mcrBC) endA1 recA1 Phi80dlacZDeltaM15 DeltalacX74 araD139 Delta(ara leu)7697 galU galK rpsL nupG lambda- tonA
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