Novus Biologicals™ Siglec-5/CD170 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human Siglec-5/CD170.

Specifications

• Assay Range: 234.38 pg/mL
• Assay Sensitivity: 234.38-15000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human Siglec-5/CD170.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD170, CD170 antigen, CD33 antigen-like 2, CD33L2OB binding protein-2, OB-BP2OB-binding protein 2, OBBP2sialic acid-binding Ig-like lectin 5, Obesity-binding protein 2, sialic acid binding Ig-like lectin 5, sialic acid-binding immunoglobulin-like lectin 5, SIGLEC-5
• Gene ID (Entrez): 8778
• Gene Symbol: SIGLEC5
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human Siglec-5/CD170 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of mouse anti-Human Siglec-5/CD170 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 685 ng of recombinant Human Siglec-5/CD170.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Siglec-5/CD170
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.