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Novus Biologicals™ Siglec-5/CD170 Antibody Pair [HRP]
SDP

Catalog No. NB009777 Shop All R&D Systems Products
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NB009777 15 Plates
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Catalog No. NB009777 Supplier Novus Biologicals™ Supplier No. NBP27936915PLATES
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Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human Siglec-5/CD170.

Specifications

Assay Range 234.38 pg/mL
Assay Sensitivity 234.38-15000 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes an antibody specific for Human Siglec-5/CD170.
Target Species Human
For Use With (Application) Sandwich ELISA
Gene Alias CD170, CD170 antigen, CD33 antigen-like 2, CD33L2OB binding protein-2, OB-BP2OB-binding protein 2, OBBP2sialic acid-binding Ig-like lectin 5, Obesity-binding protein 2, sialic acid binding Ig-like lectin 5, sialic acid-binding immunoglobulin-like lectin 5, SIGLEC-5
Gene ID (Entrez) 8778
Gene Symbol SIGLEC5
Host Species Rabbit/Mouse
Kit Contents
  • Detection Antibody: 0.2 mg/mL of rabbit anti-Human Siglec-5/CD170 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Capture Antibody: 0.5 mg/mL of mouse anti-Human Siglec-5/CD170 monoclonal antibody (in PBS, pH 7.4).
  • Standard: Each vial contains 685 ng of recombinant Human Siglec-5/CD170.
  • Quantity 15 Plates
    Regulatory Status RUO
    Storage Requirements Storage is content dependent.
    Target Siglec-5/CD170
    Technique 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
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