Siglec-G Mouse anti-Mouse, APC, Clone: SH2.1, eBioscience™ 

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3146	Invitrogen 17583380	25 μg
50-112-3147	Invitrogen 17583382	100 μg

Description

Description: The SH2.1 monoclonal antibody reacts with mouse siglec-G, which is also known as sialic acid-binding immunoglobulin-like lectin-G. Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells. Applications Reported: This SH2.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SH2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.

2 µm post-manufacturing filtered. Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.

Specifications

• Antigen: Siglec-G
• Target Species: Mouse
• Isotype: IgG1, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!

• Clone: SH2.1
• Conjugate: APC
• Applications: Flow Cytometry
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.