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Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
Supplier: Gibco™ A2494301
Description
SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool. Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.cGMP Manufacturing and Quality System
SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Store in refrigerator (2–8°C) and protect from light. Shelf life: 12 months from date of manufacture. |
|
Mammalian Cell Culture | |
Dulbecco's Modified Eagle Medium | |
Sodium Pyruvate, Non Essential Amino Acids (NEAA) | |
Sustainable packaging | |
500 mL |
Mammalian Cells | |
Liquid | |
Reduced Serum | |
No Glucose, No Glutamine, No HEPES, No Phenol Red | |
Gibco™ |
Safety and Handling
ShelfLife : 12 Months
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Product Title
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
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