Applied Biosystems™ SimpliAmp™ Thermal Cycler

Catalog No.	Warranty
A24811	Limited Warranty
A35422	5 years of total service coverage

Catalog No. A24811 Supplier Applied Biosystems™ Supplier No. A24811

Description

The SimpliAmp Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you've come to expect from Applied Biosystem instruments.

The SimpliAmp Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you've come to expect from Applied Biosystem instruments. It is elegantly designed to deliver quiet cycling and features an innovative, safe heated lid and a large LCD color touchscreen.

Features of the SimpliAmp Thermal Cycler include:

• Optimize PCR temperature with precision—VeriFlex blocks provide three independent temperature zones for precise temperature control
• 8-inch color touchscreen helps save time—the large, responsive, and easy-to-read interface simplifies instrument operation
• WiFi-enabled for remote monitoring—conveniently monitor with PCR Essentials mobile app
• Simple lid design protects samples—the innovative heated lid is designed to protect samples by automatically opening before cycling if not properly closed
• Ready-to-use USB drive for easy data backup—on-screen prompts simplify USB back-up
• Thermal simulation modes—available library of simulation modes allows experiments with the same ramp rates as previous or different instruments

Applied Biosystems PCR plastics

Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment. To find the best match please visit our Plastics Selection Guide.

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Specifications

• Thermal Accuracy: ±0.25°C (35°C to 99.9°C)
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Equipment): GeneAmp 2700, GeneAmp 9700, Veriti Thermal Cycler, 2720 Thermal Cycler
• Thermal Uniformity: <0.5°C (20 sec. after reaching 95°C)
• Dimensions: 18.1 x 9.5 x 8.3 in. (46 x 24 x 21 cm)
• Capacity: 96-well
• Voltage: 100/240 V
• Display Type: 8 in. Color TFT LCD Touchscreen
• Product Type: Thermal Cycler
• Warranty: Limited Warranty
• Thermal Range: 0°C to 100°C
• User Interface: USB, Wi-Fi, Ethernet
• Wattage: 600 W
• Max. Ramp Rate: 4°C/sec. (Block), 3°C/sec. (Sample)
• No. of Programs: >1000
• Quantity: 1 Instrument
• Weight: 18.3 lb. (8.3 kg)
• Block Configurations: Veriflex Block

Frequently Asked Questions (FAQs)

Why are my PCR tubes crushed/deformed after my PCR run?

The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:

Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.

Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).

Is there wireless connectivity with the SimpliAmp Thermal Cycler?

Like the ProFlex PCR System, the SimpliAmp Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.

How do I program an infinite hold in the SimpliAmp Thermal Cycler?

To add an infinite hold, select the infinity symbol for program.

How do the VeriFlex Blocks work on the SimpliAmp Thermal Cycler?

The SimpliAmp Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:

- PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.

What is the Auto Delta feature on the SimpliAmp Thermal Cycler?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

What is the maximum block ramp rate of the SimpliAmp Thermal Cycler?

The maximum block ramp rate of the SimpliAmp Thermal Cycler is 4 degrees C/sec.

What types of plastics can I use with the SimpliAmp Thermal Cycler?

The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html).

What types of blocks are available on the SimpliAmp Thermal Cycler?

We offer only a 96-well standard (0.2 mL) block that is not interchangeable.

Why does the SimpliAmp Thermal Cycler starter kit contain two different types of trays?

The two different types of trays are for use with different tubes:

-MicroAmp 96-well Tray/Retainer Set for Veriti Systems (Cat. No. 4381850) - this tray is for use with 0.2 mL single tubes without caps (Cat. Nos. N8010533, N8011533), 0.2 mL 8-tube strip with attached dome caps (Cat. No. A30589), and 0.2 mL tube strips without caps (Cat. No. 4316567)
-MicroAmp 96-well Tray for VeriFlex Blocks (Cat. No. 4379983) - this tray is for use with 0.2 mL single tubes with caps (Cat. Nos. N8010540, N8011540)

What is the method storage capacity for the SimpliAmp Thermal Cycler?

The storage capacity for the SimpliAmp Thermal Cycler is approximately 5.9 GB. The run methods and run records stored use most of the memory. It can store 1.2 million run methods.

How well does the SimpliAmp Thermal Cycler perform with a 4 degrees C hold?

The SimpliAmp PCR System can be used with a 4 degrees C hold. Further, our in-house QC testing requires the passing of a “no leak” test make sure that any accumulated water (due to condensation when the block temperature is set to low temperature for a long period of time) will not leak into the instrument causing electronic failure.

Can I purchase the sample block for the SimpliAmp Thermal Cycler (Cat. No. A24811) as a standalone product? Is it replaceable/interchangeable?

Unfortunately, we do not sell the sample block for the SimpliAmp Thermal Cycler separately. Only the ProFlex series and the Automated series of thermal cyclers have interchangeable blocks that we sell separately.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

How can I monitor my thermal cycler remotely without being on the Cloud?

The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)

Which thermocyclers can be controlled using the Thermal Cycler Fleet Control Software?

The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.