Molecular Probes™ Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and SYTOX™ Green Dyes, for flow cytometry

Description

Single Channel Dual 384-Well Temperature Verification Kit for PCR 9700 System, 0.02mL

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent Alexa Fluor™ 488 dye and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show brighter green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the FL1 channel of a flow cytometer, freeing the other channels for detection of other fluorescent colors.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Quantity: 1 kit
• Format: Tube
• Product Type: Dead Cell Apoptosis Kit
• Content And Storage: Contains 1 vial of annexin V, Alexa Fluor™ 488 conjugate (250 μL), 1 vial of SYTOX™ green (100 μL), and 1 bottle of annexin binding buffer (5X solution, 50 mL).
  
  Store in refrigerator (2–8°C) and protect from light.
• Flow Cytometer Laser Lines: 488
• Excitation/Emission: SYTOX™ green: 503⁄524, Alexa Fluor™ 488: 499⁄521
• No. of Reactions: 50
• Shipping Condition: Wet Ice
• Product Line: Alexa Fluor™, SYTOX™
• For Use With (Application): Flow Cytometry
• Conjugate: Alexa Fluor 488, SYTOX Green
• For Use With (Equipment): Flow Cytometer

For Research Use Only. Not for use in diagnostic procedures.