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Applied Biosystems™ TaqMan™ SNP Genotyping Assays
Description
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'‐nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). Assays contain one probe labeled with VIC or Cyanine 5 dye to detect the Allele 1 sequence; the second probe is labeled with FAM or ABY dye to detect the Allele 2 sequence.
Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
- Tested—all human SNP genotyping assays ordered with VIC/FAM dye labels are functionally tested to ensure allelic discrimination. Assays ordered with Cyanine 5/ABY dyes are not functionally tested.
- Multiplexing ready—TaqMan assays are now available with with VIC/FAM or Cyanine 5/ABY formats to support simultaneous detection of two sets of SNPs (two pairs of alleles).
Approximate ship time
4–6 days in North America and 6–10 days in Europe (VIC/FAM); 4-10 days in North America, 6-14 days in Europe (Cyanine 5/ABY)
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (singleplex) or TaqPath ProAmp Master Mix (multiplex) (sold separately).
All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355) or TaqPath ProAmp Master Mix (Cat. No. A30865)
*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.
Specifications
Specifications
| Concentration | 80X |
| Content And Storage | •1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers). •Store at -15°C to -25°C. |
| Detection Method | qPCR |
| Form | Liquid |
| Format | Tube |
| PCR Method | qPCR |
| Reaction Speed | Fast |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne, StepOnePlus, ViiA 7 System, QuantStudio Absolute Q Digital PCR System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals. |
| Internal Probe Modification | Cyanine 5 - MGB probe detects Allele 1, ABY - MGB probe detects Allele 2 |
| Label or Dye | ABY, Cy5 |
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For Research Use Only. Not for use in diagnostic procedures.
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