SK-BR-3 (human breast adenocarcinoma) Whole cell lysate, Non-denatured; Abnova

Description

• Tissue: Breast
• Host: Human
• Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

• For Use With (Application): Immunoprecipitation, Western Blot
• Tissue: Breast
• Description: Whole cell lysate (non-denatured)
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Quantity: 200 μg
• Storage Buffer: In modified RIPA Lysis Buffer.
• Host Species: Human
• Concentration: 5 mg/mL
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.