Thermo Scientific™ SkanIt™ Software for Microplate Readers

Catalog No.	Includes
50-272-3588	SkanIt SW (Research Edition)

Catalog No. 50-272-3588 Supplier Thermo Scientific™ Supplier No. 5187139

Description

Microplate reader control and data handling is simple and intuitive with the SkanIt Software, providing excellent usability and flexibility even for the most challenging microplate assays. SkanIt software helps you to optimize your research with an easy-to-read visual workflow, effortless data analysis, and flexible exporting capabilities.

With SkanIt Software, you have full control over the instrument settings for all your Thermo Scientific™ microplate readers. SkanIt™ software supports optimal use of the instrument features, offers visual workflow setup and effortless data reduction and exporting. Operation is simple and straightforward even for new users who only require basic reading capabilities. SkanIt Software is available in two editions: a research edition for scientists working in life science research and a drug discovery edition with features for compliancy with FDA 21 CFR Part 11. SkanIt Software Upgrade Package is specifically designed to support discontinued Thermo Scientific Fluoroskan, Fluoroskan FL, and Luminoskan microplate readers to enable the use of SkanIt software and USB connection between instrument and PC.

Listed below are key features of SkanIt Software

• Multi-instrument software platform compatible with all Thermo Scientific microplate readers
• Open license software for unlimited installation to multiple computers
• Research Edition for life sciences; Drug Discovery Edition with features for compliance with FDA 21 CFR Part 11
• User-interface languages available: English, French, German, Spanish, Portuguese, Italian, Russian, Japanese, and Chinese
• Robotic automation interface
• Searchable SkanIt Cloud Library with ready-to-use protocols including calculations
• Measurement sessions are easy to setup and navigate
• Intuitive Virtual Pipetting Tool assists in defining the plate layout
• Capable of endpoint, kinetic, spectral, bottom-reading multipoint, and kinetic-spectral measurements
• Build in calculations for fast, accurate data analysis including, but not limited to:
  • Parallel line analysis
  • Enzyme kinetics K_m and V_max
  • Z-factor
  • Linear and logistic curve fits with extrapolation
• Single- or multiple-wavelength measurements and multi-technology measurement capability
• Data transfer to an excel file with a single click
• Manual or automatical data export to .xlsx, .pdf, .xml, and .txt file formats
• Automatic measurement data load into a predefined Excel template file
• SkanIt Software Upgrade Package is compatible with the discontinued Fluoroskan, Fluoroskan FL, Multiskan Sky, Multiskan GO and Luminoskan microplate readers.

Specifications

• Includes: SkanIt SW (Research Edition)
• For Use With (Equipment): Varioskan LUX, Varioskan ALF, Fluoroskan, Fluoroskan FL, Luminoskan, Multiskan Sky, Multiskan SkyHigh, Multiskan FC, Multiskan GO
• Type: Software for Microplate Reader

Frequently Asked Questions (FAQs)

In the Varioskan LUX Multimode Microplate Reader, how is running a TRF spectrum by setting excitation at 335 nm and using emission range different from running a filter-based TRF assay? Why does SkanIT Software have the TRF spectrum option?

The TRF spectrum option is useful for spectral analysis, assay and label development, and optimization purpose. For example, when a new TRF chelate label is developed, TRF spectrum measurement can be used to analyze the spectra when a lot of label is loaded in the well.

When the TRF spectrum option is used, the sensitivity is not sufficient for actual TRF assays as the instrument sensitivity decreases by about 200-fold. Hence, the TRF spectrum option should not be used for normal TRF assay applications. For optimal results, the TRF spectrum option should be used for TRF spectral scanning but not for running actual TRF assays. Actual TRF assays must be performed using filter-based TRF optics in the LAT module.

If the Varioskan unit does not have TRF optics in the LAT module, TRF spectral scanning cannot be performed. In this case, TRF spectral scanning can only be performed using TRF-based filters.

What is the compatibility of SkanIt Software for Microplate Readers v7.1 with Windows 10 and 11?

In order to use SkanIt Software for Microplate Readers v7.1, your computer should meet minimum requirements as shown below:



SkanIt Software v7.1 has been updated to use Microsoft .Net Framework 4.8.

Microsoft .Net Framework 4.8 is not supported on Windows 10 1507 or 1511 versions (https://answers.microsoft.com/en-us/insider/forum/all/want-to-install-net-framework-48-in-window-10/4e60ed75-6fcc-4094-b14a-150c22139a49). If you're using Windows 10 version 1507 or 1511 and you want to install Microsoft .NET Framework 4.8, you first need to upgrade to a later Windows 10 version, i.e., version 1607 or later (https://learn.microsoft.com/en-us/dotnet/framework/install/versions-and-dependencies).

Note: Windows 10 version May 2019 onwards should have Microsoft .NET Framework 4.8 installed by default.

Is a PC included with purchase of the Multiskan Sky Microplate Spectrophotometer?

No, a PC is not included with purchase of the Multiskan Sky Microplate Spectrophotometer. Please contact your local Technical Support team for computer requirements.

Is software included with purchase of the Multiskan Sky Microplate Spectrophotometer?

Yes, SkanIt Software Research Edition version 6.0 is included with purchase of the Multiskan Sky Microplate Spectrophotometer. SkanIt Software is open-license software for unlimited installation on multiple computers.

How do I program the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer for my Quant-iT assay?

The Online Protocol Library within SkanIt Software contains several pre-written sessions. These can be used as templates and modified for use with other assays.

How do I program the Luminoskan Microplate Luminometer, Fluoroskan Microplate Fluorometer, or Fluoroskan FL Microplate Fluorometer and Luminometer for my assay such as for cell viability, cell toxicity, cell proliferation, ATP, or other assay?

The Online Protocol Library within SkanIt Software contains several pre-written sessions. These can be used as templates and modified as needed for use with other assays.

Can my Luminoskan Microplate Luminometer, Fluoroskan Microplate Fluorometer, or Fluoroskan FL Microplate Fluorometer and Luminometer perform bottom-reads for adherent cell assays?

Yes, it can. Follow the instructions for changing the measurement direction in the user manual: https://assets.thermofisher.com/TFS-Assets/BID/manuals/luminoskan-fluoroskan-fl-user-manual.pdf

Can my Luminoskan Microplate Luminometer or Fluoroskan FL Microplate Fluorometer and Luminometer read different types of luminescence assays including kinetic, dual luminescence, flash, glow, BRET, BRET2, or NanoBRET assays?

Yes, Luminoskan Microplate Luminometer and Fluoroskan FL Microplate Fluorometer and Luminometer can read any type of luminescence assay. Some will require filters.

Is there a Windows 10 compatible software for my Fluoroskan Ascent Microplate Fluorometer, Fluoroskan Ascent FL Microplate Fluorometer and Luminometer, or Luminoskan Ascent Microplate Luminometer?

If your reader's serial number begins with 374-9 followed by more digits/letters, then you can order an upgrade kit that includes a USB converter kit and SkanIt Software (Cat. No. N17646). If the serial number is 374- followed immediately by any number other than 9, then you must retain an older Windows 7, 32-bit for PC and continue using Ascent 2.6 software, as your reader's firmware is not compatible with SkanIt Software.