Invitrogen™ SMAD4 Recombinant Rabbit Monoclonal Antibody (BLR133J)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544453	Invitrogen™ MA544453	100 μL
PIMA544454	Invitrogen™ MA544454	10 μL

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-relatedprotein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Specifications

• Antigen: SMAD4
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 6.8-7.4
• Gene Accession No.: Q13485
• Gene ID (Entrez): 4089
• Immunogen: Peptide representing a region between residue 1 and 50 of Human mothers against decapentaplegic homolog 4 (SMAD4).
• Product Type: Antibody

• Clone: BLR133J
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: SMAD4
• Gene Alias: AW743858; D18Wsu70e; Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; DPC4; hSMAD4; JIP; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Madh4; mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; MYHRS; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; SMAD4
• Gene Symbols: SMAD4
• Primary or Secondary: Primary
• Purification Method: Purified