Invitrogen™ SMN1 Monoclonal Antibody (2B1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA546549

Catalog No. PIMA546549

Description

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Western blot detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa). For ICC/IF, permeabilize with 0.15% Triton.

The SMN1 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein - survival motor neuron protein. The SMN complex plays a catalyst role in the assemble of small nuclear ribonucleoproteins, the building blocks of the spliceosome. Mutations in the SMN1 gene are known to cause spinal muscular atrophy 1/2.

Specifications

• Antigen: SMN1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P97801, Q16637
• Gene Symbols: SMN1
• Immunogen: Purified human recombinant His 6 tagged-SMN protein.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Frog
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 2B1
• Conjugate: Unconjugated
• Gene: SMN1
• Gene Alias: AI849087; BCD541; component of gems 1; Gemin1; Gemin-1; SMA; SMA@; SMA1; SMA2; SMA3; SMA4; Smn; SMN1; SMN2; SMNC; SMNT; survival motor neuron 1; survival motor neuron 1 protein; survival motor neuron protein; survival of motor neuron 1, telomeric; survival of motor neuron protein; T-BCD541; TDRD16A; tudor domain containing 16A
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 20595, 6606
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov