Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 14640080

Catalog No. 50-134-75

Description

Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains. Applications Reported: This SMMS-1 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This SMMS-1 antibody has been tested by western blot on rat heart and immunohistochemistry on formalin-fixed paraffin embedded tissue (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)). This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal perfomance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

Specifications

• Antigen: Smooth Muscle Myosin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P35749, Q63862
• Gene Symbols: MYH11
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 101097693, 24582, 4629, 479836, 530050
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: SMMS-1
• Conjugate: Unconjugated
• Gene: MYH11
• Gene Alias: AAT4; AV071570; contractile protein; FAA4; KIAA0866; LOW QUALITY PROTEIN: myosin-11; Myh11; myh11 {ECO:0000312; Myosin heavy chain 11; myosin heavy chain 11, smooth muscle; myosin heavy chain 21; myosin heavy chain isoform SMA; myosin heavy chain SMA; myosin heavy chain, smooth muscle isoform; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; myosin-11; RGD:3136}; SM1; SM2; SMHC; SMMHC; smooth muscle myosin heavy chain
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Canine, Feline, Human, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.