SouthernBiotech™ Mycoplasma Detection Kit

Supplier:  SouthernBiotech™ 1310001

Catalog No. OB1310001

Includes:

• Instruction manual
• Primer set and nucleotides, lyophilized, Blue Cap: Primer/dNTP mix (deoxynucleotide triphosphates including dATP, dCTP, dGTP and dUTP).
• Sterile PCR 10X reaction buffer, 500++L, White Cap: 100mM Tris-HCL (pH 8.5), 750mM KCL, 30mM MgCL
• Positive Control DNA, lyophilized, Yellow Cap: Non-infectious PCR product from M. orale. Yields a 503-bp band.
• Internal Control DNA, lyophilized, Green Cap: Non-infectious plasmid DNA including Mycoplasma-specific primer sequences. Yields a 270-bp band.

Description

SouthernBiotech Mycoplasma Detection Kit was designed to specifically detect potential Mycoplasma contamination in cell cultures. The kit incorporates polymerase chain reaction (PCR) to amplify the conserved 16S ribosomal RNA coding region within the Mycoplasma genome, thereby providing an extensive, highly sensitive and efficient detection method. Primer sequences were carefully determined to cover three genera of Mycoplasmatales (Mycoplasma, Acholeplasma and Ureaplasma) which allows this kit to detect over 95% of potential cell culture infections.

• Fast (results are typically obtained in less than 3 hours) and easy to use PCR technology.
• Highly sensitive kit
• Can detect as little as 2-5 femtograms of Mycoplasma DNA in 100μL of test sample supernatant.
• Eukaryotic and bacterial DNA from cell culture supernatant is not amplified by the kit.
• A test cell line infected with Mycoplasma will generate a PCR product(s) between ã448-611- bp on an agarose gel, depending on the type of Mycoplasma present.
• A positive control (M.orale, 503-bp) has been included to validate that the PCR amplification process has occurred as well as to confirm the size of the PCR product obtained in test samples.
• An internal control is also provided to eliminate potential false negatives associated with PCR inhibitors.

Specifications

• Content And Storage: Instruction manual, primer set and nucleotides, lyophilized, Blue Cap, Sterile PCR 10X reaction Buffer, 500μL, White Cap, positive control DNA, lyophilized, Yellow Cap, Internal Control DNA, lyophilized, Green Cap. Store at -20°C
• Quantity: 1 Kit
• For Use With (Application): Microbial Detection