Invitrogen™ SOX10 Monoclonal Antibody (20B7), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-592-380	Invitrogen™ 14592380	25 μg
50-112-2701	Invitrogen™ 14592382	100 μg

Description

Description: The monoclonal antibody 20B7 recognizes Sox10, is a member of the Sox superfamily (SRY-related HMG-box) that contain a DNA binding domain (high-mobility group domain) and functions as a transcription factor. Initially, Sox10 expression is localized to the developing neural crest and peripheral nervous system, but later is expressed in glial progenitors and mature oligodendrocytes. Sox10 is involved in stem cell maintenance, melanocyte differentiation, as well as central and peripheral gliogenesis and neurogenesis. Sox10 regulation of downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF is dependent on expression level. Sox10 is also expressed in multipotent vascular stem cells, melanomas, and gliomas. The 20B7 antibody is reported to recognize human and rat Sox 10, and based on homology should recognize mouse Sox10. Applications Reported: This 20B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This 20B7 antibody has been tested by immunocytochemistry of formaldehyde fixed and permeabilized rat cells. This 20B7 antibody has also been tested by immunohistochemistry of human formalin-fixed paraffin embedded tissue sections using low pH antigen retreival and can be used at less than or equal to 10 μg/mL for both applications. It is recommended that the antibody be c...

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Specifications

• Antigen: SOX10
• Concentration: 0.5 mg/mL
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O55170, P56693, Q04888
• Gene ID (Entrez): 20665, 29361, 6663
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 20B7
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human, Mouse, Rat
• Gene: SOX10
• Gene Alias: cls; cls/sox10; colorless; colourless; colourless/sox10; DOM; dominant megacolon; dominant megacolon, mouse, human homolog of; golas; gos; HMG-box transcription factor Sox10; MGC15649; PCWH; Protein SOX-21; SOX10; Sox-10; sox10 protein; sox10b; Sox21; SOX9; SRY; SRY (sex determining region Y)-box 10; SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal); SRY box 10; SRY-box 10; SRY-box containing gene 10; SRY-box containing protein 10; SRY-box transcription factor 10; SRY-related HMG-box gene 10; transcription factor SOX-10; Transcription factor SOX-M; WS2E; WS4; WS4C; zgc:100757
• Gene Symbols: SOX10
• Product Type: Antibody