Invitrogen™ SPT5 Polyclonal Antibody

Catalog No.	Quantity
PIPA5143902	100 μg

Catalog No. PIPA5143902 Supplier Invitrogen™ Supplier No. PA5143902

Description

Rabbit Polyclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, mouse RAW2647 whole cell. IHC: human ovarian serous carcinoma tissue, human thyroiditis tissue, human tonsil tissue. ICC/IF: A549 cell. Flow: THP-1 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt].

Specifications

• Antigen: SPT5
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: SUPT5H
• Gene Accession No.: O00267, O55201
• Gene Alias: AL033283; AL033321; AU019549; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; etID10129.15; etID37101.15; fb37g01; fb95e05; fog; foggy; hSPT5; protein foggy; Spt5; SPT5 homolog, DSIF elongation factor subunit; SPT5H; suppressor of Ty 5; suppressor of Ty 5 homolog; suppressor of Ty 5 homolog (S. cerevisiae); Supt5; Supt5h; supt5h protein; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5; transcription elongation regulator FOGGY; wu:fb37g01; wu:fb95e05; wu:fc10f02
• Gene Symbols: Supt5, SUPT5H
• Host Species: Rabbit
• Immunogen: E.coli-derived human SPT5/SUPT5H recombinant protein (Position: Q533-A1087).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20924, 6829
• Target Species: Human, Mouse
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG