Invitrogen™ SREBP1 Polyclonal Antibody

Catalog No.	Quantity
PA1337	100 μg

Catalog No. PA1337 Supplier Invitrogen™ Supplier No. PA1337

Description

Rabbit Polyclonal Antibody

PA1-337 detects SREBP 1 in mouse and rat samples. PA1-337 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. The PA1-337 immunogen is a synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1. This sequence is identical in human and rat. PA1-337 immunizing peptide (Cat. # PEP-274) is available for use in neutralization and control experiments.

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Specifications

• Antigen: SREBP1
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: SREBF1
• Gene Accession No.: P36956, P56720, Q9WTN3
• Gene Alias: ADD1; ADD-1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; I79_010155; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBF1; SREBP1; SREBP-1; SREBP1a; SREBP-1a; SREBP1c; SREBP-1c; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element binding-protein 1; sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
• Gene Symbols: SREBF1
• Host Species: Rabbit
• Immunogen: Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20787, 6720, 78968
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

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