Invitrogen™ SSEA1 Monoclonal Antibody (MC-480), DyLight™ 650

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1022D650

Catalog No. MA1022D650

Description

MA1-022-D650 has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Specifications

• Antigen: SSEA1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with proprietary stabilizer and 0.02% sodium azide
• Gene Accession No.: P22083, Q11127
• Gene Symbols: FUT4
• Immunogen: BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgM

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: MC-480
• Conjugate: DyLight 650
• Gene: FUT4
• Gene Alias: FUTIV; Lewis X; SSEA-1
• Host Species: Mouse
• Purification Method: Affinity chromatography - MBP
• Regulatory Status: RUO
• Gene ID (Entrez): 14345, 2526
• Content And Storage: 4°C, do not freeze
• Form: Liquid