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Invitrogen™ SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Description
Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys. Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
Specifications
Specifications
| Antigen | SSEA4 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | eBioMC-813-70 (MC-813-70) |
| Concentration | 5 μL/Test |
| Conjugate | Alexa Fluor 488 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene Alias | SSEA-4; stage-specific embryonic antigen 4 |
| Host Species | Mouse |
| Purification Method | Affinity chromatography |
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For Research Use Only.
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