Invitrogen™ SSEA5 Monoclonal Antibody (8E11), DyLight™ 488

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1144D488

Catalog No. MA1144D488

Description

Immunofluorescence and flow cytometric analysis of MA1-144-D488 shows positive staining of SSEA-5 positive pluripotent cells and cultured human embryonal carcinoma NCCIT cells.

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

Specifications

• Antigen: SSEA5
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with proprietary stabilizer and 0.02% sodium azide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: 8E11
• Conjugate: DyLight 488
• Gene Alias: SSEA-5
• Immunogen: Undifferentiated H9 human embryonic stem cells.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: 4°C, do not freeze
• Form: Liquid