Invitrogen™ Human ST2 (IL-33R) ELISA Kit

Description

ELISA

The Human ST2 (IL-33R) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human ST2 (IL-33R).

IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.

Specifications

• Accession Number: DER4; FIT1; Fit-1; Fos-responsive gene 1 protein; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; Il1rl1; IL-1 RL1; IL33R; IL-33 R; interleukin 1 receptor like 1; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin1 receptor like 1; interleukin-1 receptor-like 1; Interleukin-33 receptor alpha chain; Ly84; Lymphocyte antigen 84; Protein ST2; Protein T1; sIL 33 R; soluble IL 33 Receptor; soluble IL 33 R; St2; ST2L; St2-rs1; ST2V; Ste2; T1; T1/ST2
• Assay Range: 4.7 to 300 pg/mL
• Assay Sensitivity: 4.6 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 9173
• Gene Symbol: IL1RL1
• Interassay CV: 3.5%
• Intraassay CV: 4.5
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL, Plasma 10 μL, Serum 10 μL
• Storage Requirements: 2°C to 8°C
• Target: ST2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins