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IBA LifeSciences Strep-Tactin conjugate APC; 50 µl
Supplier: IBA LifeSciences 65010001
Strep-Tactin conjugated to either R-Phycoerythrin (PE) or Allophycocyanin (APC) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer) or surface marker-specific cells (Fab-TACS/Nano-TACS) based on our Strep-tag technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin. Strep-Tactin multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.
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