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Invitrogen™ eBioscience™ Streptavidin eFluor™ 615 Conjugate
Description
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in immunohistochemistry and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.
Emit
615 nm
Excite
595 nm
Reported Application
Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections
Specifications
Specifications
| Concentration | 0.2 mg/mL |
| Conjugate | eFluor 615 |
| Quantity | 100 μg |
| Form | Liquid |
| Product Line | eBioscience |
| Product Type | Streptavidin Conjugate (fluorescent) |
| Target Molecule | Biotin |
Frequently Asked Questions (FAQs)
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.
For Research Use Only
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