Molecular Probes™ Streptavidin HRP

Catalog No.	Quantity
SNN1004	1 mg

Catalog No. SNN1004 Supplier Molecular Probes™ Supplier No. SNN1004

Description

Streptavidin HRP

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate

Specifications

• Concentration: 2 mg/mL
• Conjugate: HRP
• Quantity: 1 mg
• Content And Storage: Store in freezer (-5 to -30°C).
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Form: Liquid
• Product Type: Streptavidin Conjugate (fluorescent)
• Label or Dye: HRP (Horseradish Peroxidase)

Frequently Asked Questions (FAQs)

How is streptavidin used?

Streptavidin has a high affinity for biotin, with Kd = 1 x 10E-15 moles/L (Green, N.M. [1975] Adv. Protein Chem. 291:85). Streptavidin covalently conjugated to a reporter such as a fluorochrome (R-PE or FITC) or an enzyme (HRP or alkaline phosphatase) has utility in detecting, localizing, and quantitating biotin. Bound streptavidin conjugates can be detected either by fluorescence microscopy or by adding color producing enzyme substrate. This technique is especially useful with biotinylated antibodies. The biotinylation of antibodies is, relatively speaking, an easy procedure and the resulting product will continue to bind to its target antigen with a high degree of specificity and avidity. However, biotin itself cannot be readily detected. Biotin becomes highly apparent once streptavidin conjugated to a reporter is added.

Each streptavidin molecule can bind up to 4 biotin molecules. This feature is potentially useful for cross-linking biotinylated molecules.

For Laboratory Use