Invitrogen™ STRO-1 Monoclonal Antibody (STRO-1), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA528635

Catalog No. PIMA528635

Description

This antibody recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.

Specifications

• Antigen: STRO-1
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: TBS with 0.2% BSA and 15mM sodium azide; pH 8
• Host Species: Mouse
• Purification Method: Size-exclusion chromatography
• Regulatory Status: RUO
• Target Species: Human, Rat, Rabbit
• Product Type: Antibody
• Isotype: IgM λ

• Applications: Flow Cytometry
• Clone: STRO-1
• Conjugate: APC
• Gene Alias: STRO1
• Immunogen: Human CD34 positive bone marrow cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid