Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Catalog No.	Quantity
PI24510	500 mg
PIA39269	10 x 2 mg
PI24525	5 g

Catalog No. PI24510 Supplier Thermo Scientific™ Supplier No. 24510

Description

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce Sulfo-NHS is pure N-hydroxysulfosuccinimide, the essential compound needed to prepare water-soluble, amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.

Highlights:

• Forms an acyl amino ester with carboxyl groups when used with the EDC
• Sulfo-NHS-activated molecule reacts efficiently with primary amines, resulting in stable amide bond
• Allows for two-step EDC reactions between carboxyl- and amine-containing molecules
• Sulfo-NHS-activated molecule can be stabilized by drying, allowing manufacture of amine-reactive probes and surfaces
• Reactive groups: Sulfo-NHS
• Reactive toward: amino groups (upon activation of carboxylates)

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Specifications

• Chemical Reactivity: Amine-Carboxyl
• Cleavable: No
• Description: Sulfo-NHS
• Molecular Weight (g/mol): 217.13
• PEGylated: No
• Content And Storage: Upon receipt store at 4°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Carbodiimide
• Spacer: Short (<10 Å)
• Form: Powder
• Quantity: 500 mg
• Solubility: Water
• Format: Standard, Single-use, Premium-grade
• Water Soluble: Yes
• Product Type: Crosslinker

Frequently Asked Questions (FAQs)

Can NHS and EDC stock solutions be made for long-term storage before use?

No. EDC is very unstable in aqueous environments and must be dissolved immediately before use. NHS and Sulfo-NHS are relatively stable in solution but best results are obtained when they are dissolved immediately before use. Store these compounds desiccated at 4°C.

Are there any substances that interfere with NHS reactions?

Yes. Except for the intended targets, reactions must not contain carboxyl or amine compounds. Thus, Tris, glycine, lysine, ethanolamine or other amine- containing buffers must be avoided.

What is the difference between NHS and Sulfo-NHS?

Sulfo-NHS is the sulfonate sodium salt of NHS; it is water-soluble, but not membrane-permeable. NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical. Sulfo-NHS is chosen for its ability to confer better solubility to the activated compound and/or to control its membrane permeability.

How are NHS and Sulfo-NHS used?

These compounds are used to modify a carboxyl group to form an amine-reactive ester. This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat. No. 22980, 22981, 77149, A35391). EDC causes a dehydration reaction between the carboxyl and the NHS hydroxyl group, giving rise to an NHS-ester-activated molecule. The activated molecule can then be reacted spontaneously with a primary amine-containing molecule. Although the carboxyl-molecules can be made to react directly with amines using EDC, the reaction is much more efficient with NHS because a stable intermediate is created. In fact, molecules that are activated as NHS esters can be dried and stored for later reaction to amine-containing targets.

What are NHS and Sulfo-NHS used for?

These compounds are used in conjunction with the crosslinker EDC (Cat. No. 22980, 22981, 77149, A35391) to synthesize amine-reactive labeling reagents, crosslinkers and conjugation compounds. Any compound containing a carboxylic acid (-COOH), such as a protein, or biotin or peptide, can be activated with NHS or Sulfo-NHS to form an NHS ester that will spontaneously react to form covalent amide linkages with proteins and other molecules that contain primary amines (-NH2).

Can you provide the shelf-life for Sulfo-NHS (N-hydroxysulfosuccinimide)?

Sulfo-NHS (N-hydroxysulfosuccinimide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Can you provide the shelf life for Sulfo-NHS (N-hydroxysulfosuccinimide)?

Sulfo-NHS is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

For Research Use Only. Not for use in diagnostic procedures.