Invitrogen™ Super AquaBlue ELISA Substrate

Catalog No.	Quantity
50-112-2023	100 mL
50-184-93	500 mL

Catalog No. 50-112-2023 Supplier Invitrogen™ Supplier No. 00420356

Description

Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.

• Highly sensitive and extremely stable (18 months at room temperature or 24 months at 4°C).
• Engineered for convenience as a single component (no additional H₂O₂ or other components are necessary)
• Suitable for endpoint reactions, but is also ideal for kinetic assays; stopping the reaction with acid stop solution does not change the color or absorption properties of the blue-green end product

Specifications

• Product Type: ELISA Substrate
• Quantity: 100 mL
• Detection Method: Colorimetric
• Substrate Properties: Chemical Substrate
• Substrate Type: HRP (Horseradish Peroxidase)

Frequently Asked Questions (FAQs)

What are the differences between TMB and Super AquaBlue?

TMB is a faster developing, stronger substrate that yields greater amplification/sensitivity/background. The use of an acid stop solution produces a yellow end product read at 450nm. Super AquaBlue is a slower developing blue/green substrate particularly useful for kinetic studies.

What other reagents will I need to run an ELISA using the antibody pairs?

When working with our matched pairs or uncoated ELISA kits, please refer to the user guide provided with your antibody-matched pair for kit-specific guidance. When designing your own ELISA, please utilize our ELISA Builder tool for reagent selection as well as our ELISA Development and Optimization pages for futher guidance.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.