TOPOGEN INC Supercoiled pHOT-1 DNA Plasmid

Supplier:  TOPOGEN INC TG20303

This plasmid is ideal for assaying any topoisomerase. pHOT-1 is relatively small which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher yields. It has one additional feature for researchers who wish to assess human topo I cleavage at a single sequence: we have included the human topoisomerase I preferential binding site (the hexadecameric sequence described by Westergaard and co-workers, see Bonven et al., 1985) that is derived from the tetrahymena ribosomal gene repeat. This construct is useful as a topo I cleavage substrate since the enzyme efficiently cleaves at the hexadecameric site in the absence of camptothecin. This allows the investigator to rigorously evaluate new potential topo I inhibitors because a single background cleavage is a built-in control.

Catalog No. NC2216101