Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

Provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR)

Supplier:  Invitrogen™ 11752050

Catalog No. 11-752-050

Description

• The simple, time-saving reaction set-up uses just two tubes: a 2X Reaction Mix and an Enzyme Mix.
• Enzyme mix SuperScript III Reverse Transcriptase, included in the RT Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42° to 60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
• Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
• RNaseOUT Recombinant Ribonuclease Inhibitor, also included in the enzyme mix, is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation

Reaction mix The 2X RT Reaction Mix includes oligo(dT)₂₀, random hexamers, MgCl₂, and dNTPs in a buffer formulation that has been optimized for qRT-PCR. E. coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA:RNA hybrid molecule after first-strand synthesis. This has been shown to increase sensitivity in qRT-PCR. Using SuperScript III First-Strand Synthesis SuperMix This SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT-PCR, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1μg of total RNA. Reagents are provided for 50 or 250 RT reactions of 20μL each. Optimal reaction temperature: 50°C.

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • 2X RT Reaction Mix (500 μL)
  • RT Enzyme Mix (100 μL)
  • E. coli RNase (50 μL)
  
  Store at –20°C.
  
  

• Format: Tube
• PCR Method: 2-step RT-qPCR
• Technique: Reverse Transcription
• Reverse Transcriptase: SuperScript III
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 50 Reactions
• Reagent Type: Reverse Transcription
• Volume: 20 μL

• Detection Method: Primer-Probe
• GC-Rich PCR Performance: High
• Reaction Speed: 30 min.
• Optimal Reaction Temperature: 50°C
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Master Mix
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.