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Invitrogen™ SuperScript™ IV One-Step RT-PCR System

Enabling faster, better RT-PCR.

Supplier:  Invitrogen™ 12594100


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Catalog No. 12-594-100

  • $955.65 / Each of 1

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    Reg : $1,024.00

    The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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  • SuperScript IV RT Mix, 12.5µL
  • 2X Platinum SuperFi RT-PCR Master Mix, 625µL
  • Nuclease-free water, 1.25mL



The Invitrogen™ SuperScript™ IV One-Step RT-PCR System combines the high processivity Invitrogen™ SuperScript™ IV Reverse Transcriptase (RT) and the high-fidelity Invitrogen™ Platinum™ SuperFi™ DNA Polymerase to provide unmatched one-step RT-PCR performance. Using this convenient one-step formulation, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube, using gene-specific primers.

  • Two-phase, hot-start activation mechanism for high specificity, yield, and room-temperature setup
  • Superior performance with sensitivity down to 0.01pg of RNA, target length up to 13.8kb, and the fastest one-step RT-PCR protocol
  • Reliable target detection even with RNA samples of suboptimal purity


• SuperScript IV RT Mix (50 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –20°C.

1-step RT-PCR
10 min.
45°C to 60°C
PCR Amplified cDNA
100 Reactions
Reverse Transcription
Gel Electrophoresis
Platinum SuperFi
1-step RT-PCR
SuperScript IV
Dry Ice
Built-In Hot Start
Master Mix
Up to 14 kb


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