Invitrogen SuperScript IV Reverse Transcriptase
A proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Manufacturer: Invitrogen 18090010
Invitrogen SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.SuperScript IV Reverse Transcriptase (RT) is significantly improved over SuperScript III in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity. SuperScript IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently.
SuperScript IV RT is the top choice for all RT-PCR and qRT-PCR applications in the SuperScript product family. We recommend it especially when reproducibility and reliability is the primary concern and when inhibitors in the RNA sample may interfere with cDNA synthesis, leading to biases in gene expression studies.
Features of SuperScript IV Reverse Transcriptase include:
- Significantly improved resistance to a variety of inhibitors that can interfere with cDNA synthesis
- Robust and specific cDNA synthesis in a wide range of sample types
- A faster reverse transcriptase reaction that reduces the incubation time from >50 minutes to 10 minutes
- Significantly better processivity compared to SuperScript III RT
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance and to reduce RNase H activity.
Performance and quality testing
Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
One unit of SuperScript IV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)12-18 as a template/primer.
Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 4 mM MgCl2, 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [3H]-dTTP, 0.4 mM poly(A) oligo (dT)12-18 and enzyme in 20µl for 10 min at 37°C.
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
|Store at -5 to -30°C.|
|SuperScript IV RT (2,000 units total at 200 U/µL), 10 µL; 5X RT buffer, 1 mL; 0.1 M DTT, 500 µL|
Get 30% off select Applied Biosystems or Invitrogen gene expression products with promo code 30GENEX.
Spend up to $5,000 on select gene expression products and get a matching amount at no additional cost.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only. Not for use in diagnostic procedures.