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Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

Designed for efficient cDNA synthesis and amplification directly from intact single cells (1 to 1,000) or low amounts of total RNA (2 pg to 10 ng). Kit contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format.

$1212.00 - $6790.00

Specifications

Format Kit
Polymerase Platinum SuperFi U DNA Polymerase
Starting Material Cells (1 to 1,000), or Total RNA (2 pg to 10 ng)
Reverse Transcriptase SuperScript IV Reverse Transcriptase
Storage Requirements Store at -15° to -25°C
View More Specs

Products 5
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
11-752-048
Encompass
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Invitrogen™
11752048
48 Reactions
Each for $1,212.00
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11-752-096
Encompass
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Invitrogen™
11752096
96 Reactions
Each for $2,135.00
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11-752-192
Encompass
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Invitrogen™
11752192
192 Reactions
Each for $3,685.00
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11-752-384
Encompass
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Invitrogen™
11752384
384 Reactions
Each for $6,400.00
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11-752-480
Encompass
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Invitrogen™
11752480
480 Reactions
Each for $6,790.00
Add to cart
 
Description

Description

  • Designed for efficient cDNA synthesis and amplification directly from intact single cells (1 to 1,000) or low amounts of total RNA (2 pg to 10 ng)
  • Kit contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format
  • A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy
  • Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours
  • The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR
  • Superior sensitivity for easy detection of low abundant targets from as low as single cell or 2 pg of total RNA
  • High quality cDNA for full-length transcript information with uniform coverage
  • Global preamplification compatible with downstream analysis by NGS or real-time PCR
Specifications

Specifications

Kit
Cells (1 to 1,000), or Total RNA (2 pg to 10 ng)
Store at -15° to -25°C
NGS, qPCR
Platinum SuperFi U DNA Polymerase
SuperScript IV Reverse Transcriptase
RT-PCR
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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