Invitrogen™ SuperScript V Reverse Transcriptase

Catalog No.	Quantity
19-090-200	40,000 Units
19-090-010	2000 Units
19-090-050	10,000 Units

Catalog No. 19-090-200 Supplier Invitrogen™ Supplier No. 19090200

Description

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis.

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis. Building on the advanced performance of the SuperScript™ IV reverse transcriptase, SuperScript V RT combines high sensitivity, thermostability, and processivity with improved resistance to oxidation and greater workflow flexibility.

SuperScript V RT supports reliable cDNA synthesis from low-input RNA as well as highly structured, GC-rich, degraded, and inhibitor-containing samples.

Features of SuperScript V Reverse Transcriptase include:

• Broad RNA input range—supports cDNA synthesis from 100 fg to 5 μg of RNA
• Robust performance with challenging RNA—reaction temperatures up to 65°C help transcribe highly structured and GC-rich RNA
• Fast cDNA synthesis—complete first-strand cDNA synthesis in as little as 1–10 minutes
• Improved resistance to oxidation— enhanced reagent stability supports ambient shipping and room-temperature storage for up to 6 months
• Flexible workflow—room-temperature reaction setup and stability of assembled reactions for up to 24 hours

SuperScript V RT is well suited for RT-PCR, RT-qPCR, gene expression analysis, and other applications requiring high-quality first-strand cDNA synthesis. Its enhanced reagent and assembled-reaction stability provides additional flexibility for routine, high-throughput, and automated workflows.

A DTT-free, animal origin–free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

Specifications

• Concentration: 200 U/μL
• Content And Storage: • SuperScript V Reverse Transcriptase (200 μL)
  • 5X SuperScript V Reaction Buffer (2 x 1 mL)
  
  Store all contents at:
  • -20°C for long-term storage until expiry date
  • +5°C for up to 12 months
  • Room temperature for up to 6 months
• Detection Method: qPCR, NGS, PCR
• Form: Liquid
• Format: Stand-alone Enzyme
• GC-Rich PCR Performance: High
• PCR Method: 2-step RT-qPCR, RT-PCR
• Reaction Speed: 10 min.
• Technique: Reverse Transcription
• Label or Dye: Not Applicable
• Optimal Reaction Temperature: 50°C
• Product Type: Reverse Transcription Reagent
• Quantity: 40,000 Units
• Reverse Transcriptase: SuperScript V
• Ribonuclease H Activity: Reduced
• Sensitivity: 0.1 pg total RNA
• Shipping Condition: Dry Ice, Wet Ice, Ambient
• For Use With (Application): cDNA Synthesis, RT-PCR, Real Time PCR (qPCR), Sequencing, Next-Generation Sequencing (NGS)
• Thermostability: Up to 65°
• No. of Reactions: 200 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 20 kb
• Starting Material: RNA
• Inhibitor Tolerance (Simple): High

For Research Use Only. Not for use in diagnostic procedures.