Invitrogen™ SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal

Catalog No.	No. of Reactions	Includes
50-013-29673	1250 Reactions	SuperScript V VILO Master Mix (Primer-free) only
50-013-29671	50 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
50-013-29672	500 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
11-900-003	2000 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
11-900-111	50 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water
11-900-112	500 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water
11-900-113	2000 Reactions	SuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water

Catalog No. 50-013-29673 Supplier Invitrogen™ Supplier No. 11900004

Description

Invitrogen™ SuperScript™ V VILO™ Master Mix, Primer-Free is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis with the flexibility to select your preferred priming strategy.

Invitrogen™ SuperScript™ V VILO™ Master Mix, Primer-Free is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis with the flexibility to select your preferred priming strategy. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix, Primer-Free is also available with ezDNase™ Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix, Primer-Free include:

• Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
• Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
• Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
• Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
• Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix, Primer-Free combines SuperScript V RT, RNase inhibitor, buffer, and dNTPs in a single formulation while allowing users to select and optimize primers for their specific application. This flexibility supports gene-specific primers, oligo(dT), random primers, or customized primer combinations while maintaining the convenience of a ready-to-use master mix.

SuperScript V VILO Master Mix, Primer-Free with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA.

SuperScript V VILO Master Mix, Primer-Free also features a DTT-free, animal origin–free formulation for improved sustainability and simplified reagent handling.

Specifications

• Concentration: 5X
• Content And Storage: • SuperScript V VILO Master Mix, Primer-Free (5 mL)
  
  Store all contents at:
  • -20°C for long-term storage until expiry date
  • +5°C for up to 5 months
  • Room temperature for up to 1 month
• Detection Method: qPCR, NGS, PCR
• Form: Liquid
• Format: Master Mix
• GC-Rich PCR Performance: High
• PCR Method: 2-step RT-qPCR, RT-PCR
• Reaction Speed: 10 min.
• Technique: Reverse Transcription
• Label or Dye: Not Applicable
• Optimal Reaction Temperature: 50°C
• Product Type: Reverse Transcription Reagent
• Reverse Transcriptase: SuperScript V
• Ribonuclease H Activity: Reduced
• Sensitivity: 0.1 pg total RNA
• Shipping Condition: Dry Ice, Wet Ice, Ambient
• For Use With (Application): Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis, Next-Generation Sequencing (NGS)
• Thermostability: Up to 65°
• Final Product Type: First-Strand cDNA
• No. of Reactions: 1250 Reactions
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 20 kb
• Starting Material: RNA
• Includes: SuperScript V VILO Master Mix (Primer-free) only
• Inhibitor Tolerance (Simple): High

For Research Use Only. Not for use in diagnostic procedures.