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Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Supplier:  Thermo Scientific™ A38554

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Catalog No. PIA38554

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  • Sensitivity: low-femtogram to high-attogram
  • Stability: eight hours for working solution, one year for kit at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to four hours
  • Recommended primary antibody concentration: 1:1000 to 1:5000 dilution (0.2 to 1.0 µg/mL)
  • Recommended secondary antibody concentration: 1:100000 to 1:250000 dilution (4 to 10 ng/mL)


Nitrocellulose, PVDF
20 mL
Low-femtogram to high-attogram
• Substrate Solution, 10 mL
• Stable Peroxide Solution, 10 mL

Sufficient for two mini-gel blots; 200 cm2 of membrane.

Store reagents at 2–8°C.

Western Blot
Horseradish Peroxidase
2 Mini-Gel Blots, 200 sq. cm of Membrane
Wet Ice


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For Research Use Only. Not for use in diagnostic procedures.