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Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Description
Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
SuperSignal West Dura Extended Duration Substrate characteristics include:
- Sensitivity: mid-femtogram
- Stability: 24-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 24 hours
- Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
- Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
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Specifications
Specifications
Product Type | Substrate |
Detection Method | Chemiluminescence |
Form | Liquid |
For Use With (Application) | Western Blot |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | SuperSignal™ |
Quantity | 100 mL |
Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL) |
Sensitivity | Mid-femtogram |
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For Research Use Only. Not for use in diagnostic procedures.
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