Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Supplier:  Thermo Scientific™ 34096

Catalog No. PI34096

Description

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.

Highlights:

• ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

• Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

• Quantitative – produces quantitative signal that is measurable over two orders of magnitude

• Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

• Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

• Excellent signal duration – 8 hours of usable light-output when conditions are optimized

• Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 200 mL
• Substrate: Horseradish Peroxidase
• Sensitivity: Low femtogram-level
• Sufficient For: 25 mini-gel blots, 2000 sq. cm of membrane
• Content And Storage: Sufficient For: 25 mini-gel blots; 2000 cm² of membrane
  • Luminol/Enhancer, 100 mL
  • Stable Peroxide Buffer, 100 mL
  
  Store reagents at 4°C.

• Detection Method: Chemiluminescent
• For Use With (Application): Western Blot
• Product Line: SuperSignal™
• Conjugate: HRP
• Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
• Signal Duration: 6 to 8 hr.
• Kit Contents: Stable Peroxide Buffer, 100mL, Luminol/Enhancer, 100mL